Comparison of API 20E and invA PCR for Identification of Salmonella enterica Isolates from Swine Production Units

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Comparison of API 20E and invA PCR for identification of Salmonella enterica isolates from swine production units.

API 20E and invA PCR were evaluated for the identification of Salmonella enterica isolates from swine farms. API 20E had the highest agreement with other tests at the 99.9% likelihood level. Both tests had 100% sensitivity and 96% specificity compared to 16S rRNA sequencing. Compared to serotyping, both tests had 96% sensitivity; specificity was 86% for API 20E and 79% for invA PCR.

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The comparison of PCR technique and API-20E kit with the conventional biomedical methods for the identification of Salmonella species in laboratory

Abstract Bachground and objectives: Salmonella is one of the most important agents of gastrointestinal infection and diarrhea in our country. Misdiagnosis of these bacteria leads to cure failure. The aim of this study was to make a comparison between PCR and the API-20E and conventional biochemical tests carried out for the identification of Salmonella. Material and Methods: In this study 470 s...

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Molecular characterization of multidrug-resistant Salmonella enterica subsp. enterica serovar Typhimurium isolates from swine.

As part of a longitudinal study of antimicrobial resistance among salmonellae isolated from swine, we studied 484 Salmonella enterica subsp. enterica serovar Typhimurium (including serovar Typhimurium var. Copenhagen) isolates. We found two common pentaresistant phenotypes. The first was resistance to ampicillin, chloramphenicol, streptomycin, sulfamethoxazole, and tetracycline (the AmCmStSuTe ...

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Comparison of five repetitive-sequence-based PCR typing methods for molecular discrimination of Salmonella enterica isolates.

Five repetitive-element PCR (rep-PCR) techniques [primer sets ERIC1R-ERIC2 and REP1R-REP2I and primers ERIC2, BOXA1R, and (GTG)5] were evaluated for the discrimination of Salmonella enterica isolates at the serotype level. On the basis of number, even distribution over the whole fingerprint, and clarity of bands in the fingerprints, the enterobacterial repetitive intergenic consensus (ERIC) pri...

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 2006

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.00972-06